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cell apoptosis detection kit  (Beyotime)


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    Structured Review

    Beyotime cell apoptosis detection kit
    10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
    Cell Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+apoptosis+detection+kit/Apoptosis+Inducers+Kit/pmc13084373-53-4-20
    Average 99 stars, based on 128 article reviews
    cell apoptosis detection kit - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion"

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.03.037

    10 wt% PCL/nHA enhances cell proliferation and reduces apoptosis via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
    Figure Legend Snippet: 10 wt% PCL/nHA enhances cell proliferation and reduces apoptosis via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

    Techniques Used: Cell Culture, Concentration Assay, Control

    Piezo1-mediated Ca 2+ signaling in PCL/nHA-Driven cell behaviors. (A) Fluorescence imaging of intracellular calcium ion influx in L929 cells on PCL and PCL/nHA surfaces. (B) Fluorescence quantification of calcium ion influx in A. (C) Fluorescence imaging of intracellular calcium ion influx in HSFs on PCL and PCL/nHA surfaces. (D) Fluorescence quantification of calcium ion influx in C. (E) Fluorescence imaging assessment of intracellular ROS levels in L929 cells on PCL and PCL/nHA samples. (F) Effects of PCL samples with different nHA contents on the mitochondrial membrane potential of L929 cells. (G) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (H) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in HSFs cultured on PCL and PCL/nHA surfaces. (I) Assessment of the expression levels of apoptosis-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (J) Schematic illustration depicting the mechanisms underlying L929 cell proliferation differences. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
    Figure Legend Snippet: Piezo1-mediated Ca 2+ signaling in PCL/nHA-Driven cell behaviors. (A) Fluorescence imaging of intracellular calcium ion influx in L929 cells on PCL and PCL/nHA surfaces. (B) Fluorescence quantification of calcium ion influx in A. (C) Fluorescence imaging of intracellular calcium ion influx in HSFs on PCL and PCL/nHA surfaces. (D) Fluorescence quantification of calcium ion influx in C. (E) Fluorescence imaging assessment of intracellular ROS levels in L929 cells on PCL and PCL/nHA samples. (F) Effects of PCL samples with different nHA contents on the mitochondrial membrane potential of L929 cells. (G) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (H) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in HSFs cultured on PCL and PCL/nHA surfaces. (I) Assessment of the expression levels of apoptosis-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (J) Schematic illustration depicting the mechanisms underlying L929 cell proliferation differences. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

    Techniques Used: Fluorescence, Imaging, Membrane, Expressing, Cell Culture

    Related Articles

    CCK-8 Assay:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Cell culture medium, Penicillin-streptomycin (P/S), 0.25% trypsin solution, and Serum-free cell freezing medium were obtained from Procell Life Science & Technology Co. (China). .. CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China). .. Bovine Primary antibodies targeting Piezo1, ITGB1, and Fibronectin were acquired from Cell Signaling Technology, Inc. (USA).

    Article Title: Programmable Comodification of DNA Soccer Framework with Fluorocarbon and Aptamer for Efficient siRNA Delivery
    Article Snippet: Endocytosis inhibitors including Sucrose and Colchicine were purchased from Shanghai Sangong Biotechnology, 2,6-dimethyl-β-cyclodextrin (MβCD) was acquired from Aladdin Biochemical Technology (Shanghai, China), Indomethacin was procured from Thermo Fisher Scientific (Waltham, MA, USA). .. Cell Counting Kit-8 and Cell apoptosis detection kit (Annexin V-FITC/PI) were supplied by Beyotime Institute of Biotechnology. .. Hoechst-33342 and LysoTracker Green were purchased from Invitrogen (Carlsbad, CA, USA).

    Membrane:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Cell culture medium, Penicillin-streptomycin (P/S), 0.25% trypsin solution, and Serum-free cell freezing medium were obtained from Procell Life Science & Technology Co. (China). .. CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China). .. Bovine Primary antibodies targeting Piezo1, ITGB1, and Fibronectin were acquired from Cell Signaling Technology, Inc. (USA).

    ROS Assay:

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
    Article Snippet: Cell culture medium, Penicillin-streptomycin (P/S), 0.25% trypsin solution, and Serum-free cell freezing medium were obtained from Procell Life Science & Technology Co. (China). .. CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China). .. Bovine Primary antibodies targeting Piezo1, ITGB1, and Fibronectin were acquired from Cell Signaling Technology, Inc. (USA).

    Staining:

    Article Title: Flavonols interrupt internalized bacteria hijacking cellular responses via suppressing oxidative stress induced cellular apoptotic death.
    Article Snippet: Internalized bacteria evolve multiple means to hijack cellular responses and survive in epithelial cells for causing persistent infections.. Conventional antibiotics targeting bacteria alone often fall short in eradicating such infections, prompting the exploration of host-directed therapies as promising alternatives.. Nevertheless, the potential molecular targets



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    Image Search Results


    10 wt% PCL/nHA enhances cell proliferation and reduces apoptosis via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

    Journal: Bioactive Materials

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion

    doi: 10.1016/j.bioactmat.2026.03.037

    Figure Lengend Snippet: 10 wt% PCL/nHA enhances cell proliferation and reduces apoptosis via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

    Article Snippet: CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China).

    Techniques: Cell Culture, Concentration Assay, Control

    Piezo1-mediated Ca 2+ signaling in PCL/nHA-Driven cell behaviors. (A) Fluorescence imaging of intracellular calcium ion influx in L929 cells on PCL and PCL/nHA surfaces. (B) Fluorescence quantification of calcium ion influx in A. (C) Fluorescence imaging of intracellular calcium ion influx in HSFs on PCL and PCL/nHA surfaces. (D) Fluorescence quantification of calcium ion influx in C. (E) Fluorescence imaging assessment of intracellular ROS levels in L929 cells on PCL and PCL/nHA samples. (F) Effects of PCL samples with different nHA contents on the mitochondrial membrane potential of L929 cells. (G) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (H) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in HSFs cultured on PCL and PCL/nHA surfaces. (I) Assessment of the expression levels of apoptosis-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (J) Schematic illustration depicting the mechanisms underlying L929 cell proliferation differences. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

    Journal: Bioactive Materials

    Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion

    doi: 10.1016/j.bioactmat.2026.03.037

    Figure Lengend Snippet: Piezo1-mediated Ca 2+ signaling in PCL/nHA-Driven cell behaviors. (A) Fluorescence imaging of intracellular calcium ion influx in L929 cells on PCL and PCL/nHA surfaces. (B) Fluorescence quantification of calcium ion influx in A. (C) Fluorescence imaging of intracellular calcium ion influx in HSFs on PCL and PCL/nHA surfaces. (D) Fluorescence quantification of calcium ion influx in C. (E) Fluorescence imaging assessment of intracellular ROS levels in L929 cells on PCL and PCL/nHA samples. (F) Effects of PCL samples with different nHA contents on the mitochondrial membrane potential of L929 cells. (G) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (H) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in HSFs cultured on PCL and PCL/nHA surfaces. (I) Assessment of the expression levels of apoptosis-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (J) Schematic illustration depicting the mechanisms underlying L929 cell proliferation differences. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

    Article Snippet: CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China).

    Techniques: Fluorescence, Imaging, Membrane, Expressing, Cell Culture

    In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots of Annexin V-FITC/PI staining for apoptosis analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

    Journal: International Journal of Pharmaceutics: X

    Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition

    doi: 10.1016/j.ijpx.2026.100489

    Figure Lengend Snippet: In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots of Annexin V-FITC/PI staining for apoptosis analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

    Article Snippet: After 24 h, the cells were harvested and stained with Annexin V-FITC/PI Cell Apoptosis Detection Kit (Beyotime, China) for flow cytometer analysis.

    Techniques: In Vitro, Drug discovery, Incubation, MTT Assay, Flow Cytometry, Staining, Generated